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Archived Content

Display of Difficult Targets 

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Monday, February 20



8:25 am Chairperson's Remarks

8:30 Expanding Phage Display to Include Membrane Proteins

Gregory A. Weiss, Ph.D., Professor, Departments of Chemistry, Molecular Biology & Biochemistry, University of California, Irvine

This talk will describe the scope and limitations of the phage display of membrane proteins with examples of plasma, nuclear, peripheral, single and multipass membrane proteins for binding and re-engineering studies.

9:00 Regio-Specific Synthetic Affinity Binders as Chaperones for Membrane Structure and Probes of Function

Anthony Kossiakoff, Ph.D., Otho S. A. Professor and Chair, Department of Biochemistry and Molecular Biology, Knapp Center for Biomedical Discovery, University of Chicago

Regio-specific synthetic affinity binders (sABs) are generated using competitive phage display selection strategies to capture and stabilize different functional forms of integral membrane proteins. These sABs are based on Fab scaffolds and are effective crystallization chaperones and functional probes.

9:30 Structural Investigation of GPCR Transmembrane Signaling by Use of Nanobodies

Jan Steyaert, Ph.D., Group Leader, Structural Biology Brussels, Vrije Universiteit Brussels

Last year, we generated Nanobodies that selectively recognize an active state of the human β2 adrenergic receptor. One of these nanobodies was used to obtain the high-resolution crystal structure of this complex, providing the first view of transmembrane signaling by a GPCR3.

10:00 Networking Coffee Break with Poster Viewing

10:30 Using Yeast as a Host – Its Limitations and Advantages for Biophysical and Structural Studies of Membrane Protein

Anne S. Robinson, Ph.D., Professor, Chemical Engineering, University of Delaware

I will highlight our studies of G-protein coupled receptors, in which we try to understand how the proteins form and insert into the membrane of S. cerevisiae. We also use a variety of techniques to characterize the proteins in membrane-mimetic environments.

11:00 Baculovirus Particles as a Source of Mammalian Integral Membrane Proteins for Phage Display Applications

Isidro Hotzel, Ph.D., Scientist, Antibody Engineering, Genentech, Inc.

A major impediment to the application of phage display technology to mammalian multispan targets is the difficulty in generating and purifying these proteins. We describe the use of baculovirus particles as a source of multispan proteins for phage display applications.

11:30 Generation of Fully Human Antagonistic Antibodies against GPCR Targets

Sergej Kiprijanov, Ph.D., Vice President, Research & Pre-Clinical Development, R&D, Affitech AS

Using antibody phage display and Cell-Based Antibody Selection (CBAS™) technology, we generated a panel of antagonistic antibodies against GPCR targets involved in cancer progression and inflammation. Data showing applicability of the GPCR targeting antibodies for treatment of cancer, inflammatory and autoimmune diseases will be presented.

12:00 pm Close of Symposium

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